PULSED ELECTROCHEMICAL SENSOR

摘要

A method of detecting a biological affinity agent, comprising the steps of: providing a sensor comprising an antibody or other affinity reagent immobilised on a conductive substrate; the sensor further comprising one or more working electrodes, a counter- electrode and a reference electrode; contacting the sensor with an analyte containing the affinity agent; allowing the affinity agent to bind with the reagent; applying a voltage pulse to the sensor to polarise the sensor; disconnecting the voltage from the sensor and allowing the sensor to equilibrate in open circuit; measuring the rate of potential relaxation at the sensor; and using the measured rate of relaxation to calculate the amount of the affinity agent.