Eukaryotic expression system and use thereof

摘要

Provided are a eukaryotic expression system and its applications. The eukaryotic expression system has a recombinant plant cell. The recombinant plant cell includes a first vector and a second vector. The first vector expresses a fusion protein containing an Asia tospoviral common epitope. The fusion protein containing Asia tospoviral common epitope consists of an amino acid sequence as set forth in SEQ ID NO. 1, and a predetermined protein fragment connecting to the Asia tospoviral common epitope. The above eukaryotic expression system is useful for monitoring the interaction between proteins via use of a specific peptide to tag the predetermined protein and demonstrates high sensitivity and stability.

主权项

1. A eukaryotic expression system, comprising: a recombinant plant cell that includes:
1) a first vector that expresses a fusion protein consisting of: a) an Asia tospoviral common epitope consisting of an amino acid sequence as set forth in SEQ ID NO: 1; and b) a predetermined protein fragment of Zucchini yellow mosaic virus coat protein (ZYMV CP), wherein said predetermined protein fragment is connected to said Asia tospoviral common epitope; and 2) a second vector that expresses a target protein that binds with the predetermined protein fragment, wherein the target protein is ZYMV HC-Pro that binds to said ZYMV CP.