Scanning multifunctional particles

摘要

The present invention provides, among other things, methods and systems for characterizing multifunctional objects using a flow-through device, such as, a flow cytometer. In some embodiments, an inventive method according to the present invention includes one more steps of (a) interrogating a plurality of objects (e.g., particles), wherein each individual object (e.g., particle) comprises one or more interrogation regions detectable as a sequence of events; (b) recording multiple events, wherein each individual event corresponds to each individual interrogation region detectable above a pre-determined triggering threshold; (c) grouping the recorded multiple events, and (d) characterizing the plurality of objects based on the grouped events.

主权项

1. A method for characterizing a multifunctional particle comprising:
(a) interrogating the multifunctional object particle with a flow cytometer, wherein the multifunctional particle comprises
(i) comprising at least one coding region and(ii) at least one probe region,wherein the at least one coding region and the at least one probe region are each doped with a triggering entity that is detectable above a pre-determined triggering threshold,the at least one coding region and the at least one probe region being detectable by the flow cytometer as a sequence of discrete events above the pre-determined triggering threshold, andwherein at least one non-detectable region separates the at least one coding region and the at least one probe region; (b) detecting and recording, by the flow cytometer, the sequence of discrete events above the pre-determined triggering threshold, wherein each discrete event is designated by one or more numerical values indicative of a measured fluorescence and/or scatter signal; and (c) characterizing the sequence of discrete events based on the numerical values thereby characterizing the coding and probing regions of the multifunctional particle.