METHOD FOR PIG CMAH GENE SPECIFIC KNOCKOUT BY MEANS OF CRISPR-CAS9 AND SGRNA FOR SPECIALLY TARGETING CMAH GENE

摘要

Provided are a method for pig CMAH gene specific knockout by means of CRISPR-Cas9 and sgRNA for specially targeting a CMAH gene. A target sequence of the sgRNA for specially targeting the CMAH gene satisfies a 5'-N(20)NGG-3' sequence arrangement rule, wherein N(20) represents 20 consecutive bases, and N represents A or T or C or G; the target sequence on the CMAH gene is unique, and is located at a 5-exon coding region of an N end of the CMAH gene or a junction position with adjacent introns.