| 摘要 |
Aspects of this invention includes systems for recombinant gene expression and methods for their use to create mammalian cell lines capable of expressing high levels of convected genes. The systems include an expression vector and a host cell, and comprises the following components: (1) a target gene stabily maintained in the host cell, either on a multicopy episome or as an integrated and possibly amplified sequence; and (2) a heterologous RNA polymerase that specifically transcribes the target gene. Systems may also comprise (3) additional regulatory elements on the vector that direct transcription initiation and termination, and mRNA nucleocytoplasmic transport and translation; and/or (4) a selectable marker on the vector that allows derivation of a transduced cell population. The combination of multiple copies of the target gene template, a dedicated system for transcription, and signals mediating efficient RNA transport and translation can provide for continuous and sustainable expression of target genes at high levels suitable for large-scale production of biological products.
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