REAL-TIME TRACKING AND CORRELATION OF MICROSPHERES

摘要

Methods and apparatuses for tracking and correlating particles include an optical detector that captures a first and a second image of the particles. A video detector is used to capture a plurality of video frames of the particles. The video detector captures the video frames of the particles at a rate faster than the rate at which images are captured by the optical detector to track the movement of particles. A first image position of a particle in the first image of the particles is identified, and then the first image position of the particle is correlated to a second image position of the particle in the second image using the plurality of video frames.

主权项

1. A method for correlating a position of a droplet in a first image with a position of the droplet in a second image, comprising:
capturing, using an optical detector, a first and a second image of one or more droplets; capturing, using a video detector, a plurality of video frames of the one or more droplets; identifying, using a processor, a first image position of a droplet in the first image of the one or more droplets; transforming the identified first image position of the droplet in the first image from a position in an image coordinate system to a position in a video frame coordinate system to identify a first video frame position of the plurality of video frames; identifying a droplet with a position in the identified first video frame that is closest to the first video frame position; tracking the movement of the droplet from the first video frame to a second video frame to locate a second video frame position of the droplet in the second video frame; and inverse transforming the second video frame position in the video frame coordinate system to a position in the image coordinate system to identify a second image position of the droplet in the second image.