| 摘要 |
This invention allows efficient tagging and normalization of DNA molecules prior to pooling and characterization using parallel DNA sequencing (e.g., commercially available 454 sequencers). The invention provides novel ways to process independent DNA samples (sources) so that similar numbers of molecules from each source are represented in the pool (i.e., the pool is normalized for representation among the sources). These approaches would save researchers time and energy relative to approaches currently available. In other embodiments, the invention provides novel ways to process large numbers of independently derived DNA samples that can be uniquely tagged in ways that allow the source of the DNA (e.g., an individual) to be tracked. The invention also provides novel ways for processing DNA to consistently obtain various portions of the genome to compare DNA sequences and directly measure mutations using state of the art massively parallel DNA sequencing (e.g., commercially available 454 sequencers and others. The invention is useful for the production of diagnostic assays for individuals prior to reproduction (e.g., cancer survivors who wish to procreate) and also for diagnostics of cancer (thus affecting the choice of treatment for cancer).
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