| 主权项 |
1. A nonnaturally occurring nucleic acid encoding an insecticidal protein comprising:
(a) the nucleotide sequence set forth in SEQ ID NO: 1; (b) the nucleotide sequence set forth in SEQ ID NO: 2; (c) the nucleotide sequence set forth in SEQ ID NO: 3; (d) the nucleotide sequence set forth in SEQ ID NO: 4; (e) the nucleotide sequence set forth in SEQ ID NO: 5; (f) the nucleotide sequence set forth in SEQ ID NO: 6; (g) a nucleotide sequence that is at least 95% identical to the nucleotide sequence of any one of SEQ ID NOs: 1 to 6; (h) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 7; (i) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 8; (j) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 9; (k) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 10; (l) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence that is at least 95% identical to the amino acid sequence set forth in any one of SEQ ID NOs: 7-10; (m) a nucleotide sequence that is complementary to the nucleotide sequence of any one of (a) to (l) above; (n) a nucleotide sequence that hybridizes to the nucleotide sequence of any one of (a) to (m) above under stringent hybridization conditions; (o) a functional fragment of any one of (a), (c), (d), (f), (g), (h) and (j) above, wherein the functional fragment encodes an insecticidal δ-endotoxin; (p) a functional fragment of any one of (a), (c), (d), (f), (g), (h) and (j) above, wherein the functional fragment encodes a polypeptide that comprises an N-terminal helical bundle domain, a central beta-sheet domain and a C-terminal beta-sandwich domain; and/or (q) a functional fragment of any one of (b), (c), (e), (f), (g), (i) and (k) above, wherein the functional fragment encodes a polypeptide the expression of which increases the expression, stability and/or activity of one or more δ-endotoxins; or (r) a nucleotide sequence of any one of (a) to (q) above that has been codon optimized for expression in a transgenic plant. |
