发明名称 |
DNA POLYMERASES WITH INCREASED 3'-MISMATCH DISCRIMINATION |
摘要 |
Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases. |
申请公布号 |
US2016230153(A1) |
申请公布日期 |
2016.08.11 |
申请号 |
US201615138029 |
申请日期 |
2016.04.25 |
申请人 |
Roche Molecular Systems, Inc. |
发明人 |
Reichert Fred;Bauer Keith;Myers Thomas W. |
分类号 |
C12N9/12 |
主分类号 |
C12N9/12 |
代理机构 |
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代理人 |
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主权项 |
1. A DNA polymerase having increased 3′-mismatch discrimination activity compared with a control DNA polymerase, wherein the DNA polymerase comprises a motif in the polymerase domain comprising
G-Y-V-X1-T-L-F-G-R-X4-X5-X6-X7-P, wherein: X1 is E or R; X4 is R or K; X5 is any amino acid other than R; X6 is Y or D; and X7 is V or I (SEQ ID NO:9). |
地址 |
Pleasanton CA US |