PCR AMPLIFICATION METHODS, PRIMERS, AND PROBES FOR DETECTING AND QUANTIFYING SULFATE-REDUCING BACTERIA

摘要

At least one nucleic acid from a sulphate-reducing bacterium may be extracted from a sample and may be amplified by a PCR amplification method in the presence of at least one primer to form an amplification product. The primer(s) may include a sequence essentially identical to SEQ ID NO:1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, and mixtures thereof. A probe may hybridize with the amplification product from the PCR amplification method where the probe includes a sequence essentially identical to SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, and mixtures thereof.

主权项

1. A PCR amplification method comprising:
amplifying at least one nucleic acid of at least one sulfur-reducing bacteria in the presence of at least one primer to form an amplification product; wherein the at least one nucleic acid is extracted from a sample prior to amplifying the at least one nucleic acid; wherein the at least one primer comprises an essentially identical sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, and mixtures thereof.