Methods and compositions for backscattering interferometry

摘要

The present invention relates to methods and compositions for enhancing backscattering interferometry (BSI) in detection of biomolecular interactions, particularly to methods and compositions for enhancing BSI utilizing label-free aptamers, and more particularly to methods and compositions for enhancing BSI utilizing high conformational change aptamers, which may change in conformation when the aptamers bind to their target molecules.

主权项

1. A non-labeled molecular beacon comprising:
a hairpin aptamer having a non-self-hybridizing loop region and self-hybridizing stem region, said hairpin aptamer comprising a non-naturally occurring sequence;wherein said self-hybridizing stem region dehybridizes upon binding of said hairpin aptamer to a target molecule to elicit a conformational change in said hairpin aptamer.