SEPARATING AGENT

摘要

The problem addressed by the present invention is to provide a novel separating medium for hydrophilic interaction chromatography useful in separating hydrophilic compounds. The hydrophilic interaction chromatography separating medium, which is formed from a support and a ligand carried by the support, is a separating medium wherein the ligand is a (meth)acrylic polymer having a constituent unit derived from the compound indicated by formula (I).;;(In formula (I): there are one or two double bonds between atoms configuring a heterocycle; X1 is selected from the group consisting of S, SCH3+, O, NH, NCH3, CH2, CHR, and CR1R2; and X2, X3, and X4 are each selected from the group consisting of N, NH, NCH3, CH2, CHR, NCH3+, CH, CR, and CR1R2; R1 and R2 are each a substituted or unsubstituted alkyl having 1-18 carbon atoms, an aryl having 6-18 carbon atoms, an alkenyl having 2-18 carbon atoms, an alkynyl having 2-18 carbon atoms, an aralkyl having 7-18 carbon atoms, an acyl having 2-18 carbon atoms, a cycloalkyl having 3-18 carbon atoms, a carboxyl, an amino, an aryloxy having 6-18 carbon atoms, an alkoxy having 1-18 carbon atoms, a halo, a hydroxy, a nitro, or a cyano. R is a substituted or unsubstituted alkyl having 1-18 carbon atoms, an aryl having 6-18 carbon atoms, an alkenyl having 2-18 carbon atoms, an alkynyl having 2-18 carbon atoms, an aralkyl having 7-18 carbon atoms, an acyl having 2-18 carbon atoms, a cycloalkyl having 3-18 carbon atoms, a carboxyl, an amino, an aryloxy having 6-18 carbon atoms, an alkoxy having 1-18 carbon atoms, a halo, a hydroxy, a nitro, or a cyano. At least two of X1, X2, X3, and X4 are not CH2, CH, CR, or CR1R2, and R3 is H or CH3.)

主权项

1. A separating medium for hydrophilic interaction chromatography, being formed from a support and a ligand supported on the support, wherein the ligand is a (meth)acrylic polymer having a constituent unit derived from a compound represented by Formula (I) below: in Formula (I), there are one or two double bonds between atoms making up a heterocyclic ring, X1 is selected from the group consisting of S, SCH3+, O, NH, NCH3, CH2, CHR and CR1R2, each of X2, X3 and X4 is selected from the group consisting of N, NH, NCH3, CH2, CHR, NCH3+, CH, CR and CR1R2 (in which each of R1 and R2 is an optionally substituted C1-18 alkyl, C6-18 aryl, C2-18 alkenyl, C2-18 alkynyl, C7-18 aralkyl, C2-18 acyl, C3-18 cycloalkyl, carboxyl, amino, C6-18 aryloxy or C1-18 alkoxy, halo, hydroxyl, nitro or cyano group, while R is a substituted or non-substituted C1-18 alkyl, C6-18 aryl, C2-18 alkenyl, C2-18 alkynyl, C7-18 aralkyl, C2-18 acyl, C3-18 cycloalkyl, carboxyl, amino, C6-18 aryloxy or C1-18 alkoxy, halo, hydroxyl, nitro or cyano group), at least two of X1, X2, X3 and X4 are not CH2, CH, CR or CR1R2, and R3 is H or CH3.