Real-time tracking and correlation of microspheres

摘要

Methods and apparatuses for tracking and correlating particles include an optical detector that captures a first and a second image of the particles. A video detector is used to capture a plurality of video frames of the particles. The video detector captures the video frames of the particles at a rate faster than the rate at which images are captured by the optical detector to track the movement of particles. A first image position of a particle in the first image of the particles is identified, and then the first image position of the particle is correlated to a second image position of the particle in the second image using the plurality of video frames.

主权项

1. A method, comprising:
capturing, using an optical detector, a first and a second image of one or more particles; capturing, using a video detector, a plurality of video frames of the one or more particles; time stamping the first and second images captured using the optical detector; time stamping the plurality of video frames captured using the video detector; identifying, using the processor, a first image position of a particle in the first image of the one or more particles; correlating, using the time stamps and a processor, the first image position of the particle in the first image to a second image position of the particle in the second image using the plurality of video frames; transforming the identified first image position of the particle in the first image from a position in an image coordinate system to a position in a video frame coordinate system to identify a first video frame position; identifying a first video frame of the plurality of video frames with a time stamp that is closest to a time stamp of the first image; identifying a particle with a position in the identified first video frame that is closest to the first video frame position; tracking the movement of the particle from the first video frame to a second video frame to locate a second video frame position of the particle in the second video frame; and inverse transforming the second video frame position in the video frame coordinate system to a position in the image coordinate system to identify a second image position of the particle in the second image.