A METHOD FOR QUANTIFYING AN ANALYTE, AND AN AUTOMATIC ANALYTICAL DEVICE CONFIGURED TO IMPLEMENT SAID METHOD

摘要

The present invention deals with a novel method for determining the amount of an analyte in a sample comprising an initial purification step, occurring in a first container, comprising the following steps of mixing the sample, a delipidation agent and magnetic particles coated with first analyte binding partners in the first container, incubating the mix, removing the unbound reagents from the mix, and eluting the bound analyte in an elution solution; a transferring step consisting transferring in a volume of the elution solution comprising the analyte from the first container to a second container; and a quantification step, occurring in the second container, consisting of quantifying the analyte in said elution solution.

主权项

1. A method for determining the amount of an analyte in a sample comprising:
a purification step comprising the following steps:a) mixing the sample, a delipidation agent and first magnetic particles coated with first analyte binding partners in the first container,b) incubating the mix contained in the first container so as to precipitate lipids contained in the sample and to bind the analyte contained in the sample to the first analyte binding partners,c) subjecting the first container to a magnetic field so as to magnetically attracting the first magnetic particles to an inner wall portion of the first container,d) removing unbound reagents from the mix contained in the first container,e) eluting the bound analyte in an elution solution so as to separate the analyte from the first analyte binding partners,
a transferring step comprising the following steps:f) subjecting the first container to a magnetic field so as to magnetically attracting the first magnetic particles to an inner wall portion of the first container,g) transferring a volume of the elution solution comprising the analyte from the first container to a second container, and
a quantification step, occurring in the second container, comprising the quantification of the analyte.