Glucosyl stevia composition

摘要

Glucosyl stevia compositions are prepared from steviol glycosides of Stevia rebaudiana Bertoni. The glucosylation was performed by cyclodextrin glucanotransferase using the starch as source of glucose residues. The compositions mainly comprise glucosyl derivatives with superior taste characteristics and can be used as sweetness enhancers, flavor enhancers and sweeteners in foods, beverages, cosmetics and pharmaceuticals.

主权项

1. A process for producing a highly purified glucosyl stevia composition, comprising the steps of:
adding starch into water to form a starch suspension; adding a mixture of α-amylase and CGTase into the starch suspension and incubating for about 0.5 to 2 hours at about 75-80° C., resulting in a liquefied starch suspension; inactivating the α-amylase by low pH heat treatment; cooling the liquefied starch suspension and adjusting the pH to about 5.5 to 7.0; adding a freely soluble form of steviol glycosides selected from the group consisting of rebaudioside B and steviolbioside into the liquefied starch suspension, resulting in a reaction mixture; adding a second batch of CGTase into the reaction mixture and incubating for about 12 to 48 hours at about 55-75° C.; inactivating the CGTase by low heat treatment; decolorizing the reaction mixture; cooling the reaction mixture and adjusting the pH to about 2.0 to 4.5; precipitating the unreacted steviol glycosides; separating the precipitated unreacted steviol glycosides crystal from filtrate; adjusting the pH of filtrate to about 5.5 to 7.0; removing impurities by contacting the filtrate with macroporous adsorbent resin and subsequently eluting adsorbed diterpene glycosides with aqueous ethanol to result in a glycoside-containing aqueous ethanol eluate; removing ethanol from the aqueous ethanol eluate, resulting in an aqueous eluate; and concentrating and drying the aqueous eluate to obtain the highly purified glucosyl stevia composition, wherein the highly purified glucosyl stevia composition comprises derivatives containing α-1,4-glucosyl residues attached to glycosidic residues of C13 position of steviol, and unmodified steviol glycosides.