CELL LINE I DERIVED FROM CANCER TISSUE AND METHOD FOR PREPARING THE SAME I

摘要

A multipotential cell line I derived from cancer tissue is provided to treat various diseases by differentiating into various types of cells including epidermal cell and nerve cell, study on the cancer development mechanism, and develop a new anticancer agent targeting proteins expressed from the same cell line. A multipotential cell line I(KCLRF-BP-00137) is derived from cancer tissue, can be cultured in a suspension state, and expresses markers of breast stem cell(CK19, CK5, CK6), a marker of breast cancer resistant drug(ABCG2), a marker of breast cancer diagnosis receptor(C-erBb2), markers of epidermal cell(CK14, vimentin, CK18) and markers of nerve cell(nestin, Tuj-1). A method for preparing the multipotential cell line I(KCLRF-BP-00137) comprises the steps of: (1) separating cancer cells from the previously separated cancer tissue; (2) culturing the separated cancer cells in a suspension medium; (3) collecting the cells in a suspension state from the cultured medium; and (4) subculturing the collected cells in a suspension medium to separate the multipotential cell line. Further, the cancer tissue is breast cancer.