METHOD AND DEVICE FOR DETECTING IN REAL TIME A SECRETED COMPOUND AND THE SECRETION TARGET, AND USES THEREOF

摘要

The present invention relates to a method for measuring in real time the secretion of a compound Co by a target Ci, said method comprising: culturing, in a liquid medium, in a culture chamber, a plurality of targets including at least one target Ci, said culture chamber comprising: (i) at least one 1st surface on which said target Ci is present, the presence of said target Ci generating a 1st signal; and (ii) at least one 2nd surface that is different from said 1st surface and not coplanar with said 1st surface, which surface is functionalised by at least one ligand specifically binding to said compound Co secreted by said target Ci, the specific bond between said ligand and said compound Co generating a 2nd signal that is distinct from said 1st signal; and detecting in real time said 2nd signal and optionally said 1st signal. The present invention also relates to devices possibly implemented in such a method.