GENE CODING LACCASE DERIVED FROM POLYPORUS BRUMALIS, LACCASE ENZYMES AND PREPARATION METHOD THEREOF

摘要

Genes encoding laccase derived from Polyporus brumalis are provided to degrade phthalates biologically as environment hormones and produce laccase having high lignin-decomposing activity, so that it is useful for production of edible mushroom having enhanced lignin-decomposing activity. A gene Pblac1 or Pblac2 encoding laccase having the amino acid sequence of SEQ ID NO:3 or SEQ ID NO:4 derived from Polyporus brumalis has the nucleotide sequence of SEQ ID NO:1 or SEQ ID NO:2. The laccase is produced by culturing Polyporus brumalis in a medium containing phthalates. The phthalates are degraded by contacting with Polyporus brumalis.