Bispecific binding molecules binding to VEGF and ANG2

摘要

Bispecific binding molecules binding to both VEGF and Ang2, preferably in the form of immunoglobulin single variable domains like VHHs and domain antibodies, pharmaceutical compositions containing the same and their use in the treatment of diseases that are associated with VEGF- and/or Ang2-mediated effects on angiogenesis are disclosed. Further, nucleic acids encoding bispecific binding molecules, host cells and methods for preparing same are also described.

主权项

1. A bispecific binding molecule comprising:
at least one VEGF-binding component, at least one serum albumin binding component, and at least one Angiopoietin-2 (Ang2) binding component, wherein said VEGF-, serum albumin and Ang2-binding components are immunoglobulin single variable domains, each immunoglobulin single variable domain consisting of four framework regions and three complementarity determining regions (CDRs), and wherein: said VEGF binding immunoglobulin single variable domain, comprises the following CDR sequences:
CDR1: SYSMG (SEQ ID NO: 450)CDR2: AISKGGYKYDAVSLEG (SEQ ID NO: 465)CDR3: SRAYGSSRLRLADTYEY (SEQ ID NO: 4) said serum albumin binding immunoglobulin single variable domain, comprises the following CDR sequences:
CDR1: SFGMS (SEQ ID NO:255)CDR2: SISGSGSDTLYADSVKG (SEQ ID NO:256)CDR3: GGSLSR (SEQ ID NO:257); and said Ang2-binding immunoglobulin single variable domain, comprises the following CDR sequences:
CDR1: DYAIG (SEQ ID NO:248)CDR2: AIRSSGGSTYYADSVKG (SEQ ID NO:249)CDR3: VPAGRLRYGEQWYPIYEYDA (SEQ ID NO:250).