| 发明名称 |
METHOD OF INCREASING BIOMASS AND LIPID CONTENT IN A MICRO-ORGANISM AND A GENETICALLY MODIFIED MICRO-ORGANISM EXHIBITING ENHANCED AUTOPHAGY |
| 摘要 |
According to an embodiment of the invention, there is provided a method of increasing biomass and lipid content in a micro-organism comprising cloning in a vector an exogenous gene sequence selected from the group comprising Atg1 gene, Atg6 gene, and Atg8 gene sequence wherein the sequence is codon optimized for said micro-organism, for inducing autophagy; introducing the vector containing the gene into the genome of the micro-organism to yield a genetically modified micro-organism; and growing the genetically modified micro-organism in suitable medium. According to another embodiment of the invention there is provided a method of increasing biomass and lipid content in a micro-organism exposed to stress, comprising treating the microorganism with an autophagy inducing agent. |
| 申请公布号 |
US2016369307(A1) |
申请公布日期 |
2016.12.22 |
| 申请号 |
US201515110683 |
申请日期 |
2015.01.09 |
| 申请人 |
RELIANCE INDUSTRIES LIMITED |
发明人 |
DAS Gautam;DASGUPTA Santanu;KUMAR Raja;PRASAD Venkatesh;DEEPAK Nanjappa;SONI Niraja;DATE Amol;SONI Badrish Ranjitlal;NAGARJUNA Pasupuleti |
| 分类号 |
C12P7/64;C07K14/405;C12N1/12;C12N15/82 |
主分类号 |
C12P7/64 |
| 代理机构 |
|
代理人 |
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| 主权项 |
1. A method of increasing biomass and lipid content in a micro-organism exposed to stress comprising:
a. cloning in a vector an exogenous gene sequence selected from the group comprising Atg1 gene, Atg6 gene, and Atg8 gene sequence wherein the sequence is at least 50% homologous with Atg1 gene, Atg6 gene, and Atg8 gene, codon optimized for said micro-organism, for inducing autophagy; b. introducing the vector containing the gene into the genome of the micro-organism to yield a genetically modified micro-organism; and c. growing the genetically modified micro-organism in suitable medium. |
| 地址 |
Maharashtra IN |
