METHOD FOR SEPARATING AND CONCENTRATING TARGET SUBSTANCE USING NOVEL CATIONIC GRAFT POLYMER

摘要

The purpose of the present invention is to provide a method for separating and concentrating a target substance as an alternative to existing cationic polymers, and a kit for implementing this method.

主权项

1. A method for separating and concentrating a target substance using a cationic graft polymer, comprising:
(1) step of bringing the cationic graft polymer into contact with a sample containing the target substance to make the target substance bound to the cationic graft polymer under a basic condition; (2) step of separating the bound complex of the cationic graft polymer and the target biomolecule; and (3) step of eluting the target substance from the bound complex, wherein the cationic graft polymer is a polyamine graft polymer obtained by polymerizing a polyamine derivative obtained by a reaction between (a) a polymer compound having at least one amino group and (b) a compound having at least one epoxy group, and (c) an ethylenically unsaturated monomer.