摘要 |
The subject of the present invention is a method for determining the hydrodynamic range of the constituents of a mixture, in which: (A) by capillary electrophoresis, the constituents of the mixture M are separated, leaving them within the capillary; (B) at one of the ends of the capillary thus obtained, containing, in various zones, the constituents separated in step (A), a marker is injected that can be detected by a detection device placed on the side facing the other end of the capillary; (C) a pressure difference is induced between the ends of the capillary so as to make the various constituents separated during step (A) and, finally, the marker migrate towards the outlet of the capillary; and (D) the hydrodynamic range of each of said constituents is determined, by analysing the Taylor dispersion produced in step (C), from the time at which the marker is detected and from the elution profile of each of the constituents. |