发明名称 |
Chimeric proteins comprising liver enriched transcription factors and nucleic acids encoding the same |
摘要 |
HNF-4 (hepatocyte nuclear factor 4) is a protein enriched in liver extracts that binds to sites required for the transcription of the transthyretin (TTR) and apolipoprotein CIII (apoCIII) genes (Costa et al., 1989; Costa et al., 1990; Leff et al., 1989). We have purified HNF-4 protein (54 kD) and isolated a cDNA clone encoding the protein. HNF-4 is a member of the steroid hormone receptor superfamily with an unusual amino acid in the conserved "knuckle" of the first zinc finger (DGCKG). This and the fact that HNF-4 does not bind significantly to estrogen, thyroid hormone or glucocorticoid response elements indicate that HNF-4 may represent a new subfamily. HNF-4 binds to its recognition site as a dimer and activates transcription in a sequence-specific fashion in nonhepatic (HeLa) cells. HNF-4 mRNA is present in kidney and intestine as well as liver but is absent in other tissues. DNA binding data suggest that HNF-4 could be identical to liver factor A1 (LF-A1), a factor previously shown to regulate the transcription of the alpha -1 antitrypsin, apolipoprotein A1 and pyruvate kinase genes.
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申请公布号 |
US6025196(A) |
申请公布日期 |
2000.02.15 |
申请号 |
US19980038217 |
申请日期 |
1998.03.11 |
申请人 |
THE ROCKEFELLER UNIVERSITY |
发明人 |
SLADEK, FRANCES M.;ZHONG, WEIMIN;DARNELL, JR., JAMES E. |
分类号 |
A61K31/70;A61K38/00;A61K39/395;A61P3/04;A61P9/00;C07K14/00;C07K14/435;C07K14/47;C07K14/705;C07K14/72;C07K16/00;C07K16/42;C07K19/00;C12N1/19;C12N1/21;C12N5/10;C12N15/09;C12N15/113;C12N15/12;C12N15/67;C12P21/08;C12Q1/68;G01N33/53;G01N33/534;G01N33/577;(IPC1-7):C12N15/00;C12P21/04;C07K1/00;C07H21/04 |
主分类号 |
A61K31/70 |
代理机构 |
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