RECOMBINATION ACTIVATING GENE 2 GENE TARGETING VECTOR, PRODUCTION OF SCID-LIKE MINIATURE PIGS BY TALEN-MEDIATED GENE TARGETING AND USE THEREOF

摘要

There are provided to a Rag-2 (Recombination activating gene 2) gene targeting vector, a method for producing SCID-like miniature pigs introduced with the vector, and a use thereof.

主权项

1. A method for producing severe combined immunodeficiency (SCID)-like miniature pigs having recombination activating gene (RAG) 2-allelic mutation, the method comprising:
inducing an allelic mutation by treating a transcription activator-like effector nuclease (TALEN) to a TALEN-recognizing sequence region as set forth in SEQ ID NO. 1 in Chromosome 2 of a pig (Sus scrofa); and producing mutant embryos by using the cells with the induced allelic mutation for somatic cell nuclear transplantation (SCNT).