| 主权项 |
1. A method for making a target polypeptide, said method comprising the steps:
a) expressing an Enterokinase-cleavable fusion polypeptide comprising a polypeptide of formula I:
Z2-X6-X5-X4-G-D-R-Z1 (I) SEQ ID NO: 1whereinZ1 is a polypeptide comprising at least 2 amino acid residues;X4 is an amino acid selected from the group consisting of E, Q, L, D, G, A, S, F, H, Y, W, T or M;X5 is an amino acid selected from the group consisting of genetically encoded amino acids other than S and I;X6 is absent or an amino acid selected from the group consisting of genetically encoded amino acids;Z2 is optionally a polypeptide or an amino acid residue;wherein said target polypeptide is Z1 in formula (I); b) contacting said Enterokinase-cleavable fusion polypeptide with an Enterokinase under conditions facilitating cleavage of said fusion polypeptide; and c) optionally isolating said target polypeptide from said cleavage reaction in b). |
