METHODS OF IMPROVED PROTEIN PRODUCTION USING MIRNAs AND SIRNAs

摘要

The invention provides a method of increasing protein production in a cell by contacting the cell with miRNA, siRNA or a combination thereof, or increasing protein production by genome editing methodologies to silence or inhibit gene expression. A screening method for obtaining such miRNA or siRNA species is also provided, as well as identification of target genes for genome editing.

主权项

1. A method of increasing production of a protein of interest in a cell comprising contacting the cell with an miRNA, siRNA or combination thereof under conditions wherein the miRNA or siRNA is incorporated into the cell, wherein an increase in production of the protein greater than that of a control cell not contacted with the miRNA or siRNA is indicative of increased protein production in the cell, thereby increasing production of the protein of interest in the cell.