TEMPERATURE-RESPONSIVE POLYMER PARTICLES IN PROTEIN SEPARATION APPLICATIONS

摘要

Disclosed is a method for isolating proteins from a solution containing the proteins, the method including: a. contacting the solution containing the proteins with crosslinked polymer particles functionalized with temperature-responsive copolymer, wherein the temperature-responsive copolymer includes a proportion of ionizable chemical groups and said contacting occurs at a temperature between 30°C and 80 °C to facilitate retention of the proteins by the crosslinked polymer particles; b. replacing the solution containing the protein with a rinse solution; c. replacing the rinse solution with a release solution effective for releasing the proteins from the crosslinked polymer particles; and d. isolating the release solution containing the protein. Further disclosed is a crosslinked hydroxylic polymer particles functionalized with temperature-responsive copolymer, wherein the temperature-responsive copolymer includes a proportion of ionizable chemical groups.