发明名称 |
BIOMARKER FOR MELK ACTIVITY AND METHODS OF USING SAME |
摘要 |
The methods of the present invention, relate to the surprising determination that the level of phosphorylation of position 406 (e.g., a serine residue) of human eukaryotic initiation factor 4B (eIF4B), or a corresponding phosphorylatable amino acid of an ortholog thereof, serves as a biomarker for MELK enzymatic (e.g., kinase) and/or oncogenic activity. The methods of the present invention further relate to the surprising determination that the level of phosphorylation of position 3 (e.g., a threonine residue) and/or position 10 (e.g., a serine residue) and/or position 11 (e.g., a threonine residue) of human Histone M3, or a corresponding phosphorylatable amino acid of an ortholog thereof, also serves as a biomarker for MELK enzymatic (e.g., kinase) and/or oncogenic activity. |
申请公布号 |
US2016291017(A1) |
申请公布日期 |
2016.10.06 |
申请号 |
US201415034955 |
申请日期 |
2014.11.12 |
申请人 |
DANA-FARBER CANCER INSTITUTE, INC. |
发明人 |
Zhao Jean;Wang Yubao |
分类号 |
G01N33/573;G01N33/68;G01N33/574 |
主分类号 |
G01N33/573 |
代理机构 |
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代理人 |
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主权项 |
1. A method of identifying an agent which inhibits kinase or oncogenic activity of human maternal embryonic leucine zipper kinase (MELK) or an ortholog thereof, comprising:
a) contacting a sample comprising i) human MELK or an ortholog thereof and ii) human eukaryotic initiation factor 4B (eIF4B) or an ortholog thereof, with the agent; and b) determining the ability of the agent to inhibit Ser-406 phosphorylation of human eIF4B or a corresponding phosphorylatable amino acid in the ortholog of human eIF4B, wherein decreased phosphorylation identifies an agent which inhibits kinase or oncogenic activity of human MELK or the ortholog thereof. |
地址 |
Boston MA US |