Screening of protein candidates

摘要

Successful application of an engineered protein as therapeutics or in other industries would require the protein to have good expression level, good biophysical properties and often desired affinity to its target. The present invention provides a method of screening large numbers of protein candidates (PCs) in all three aspects simultaneously. PCs are fused to a protein anchor, which is captured by the target/antigen. The captured PCs are evaluated for their expression levels, biophysical properties and affinities using conventional methods.

主权项

1. A method for screening of protein candidates, comprising:
a) providing fusion proteins, each fusion protein comprising one protein candidate and a protein anchor, wherein the protein anchor is an antibody or antibody fragment comprising a complementarity determining region (CDR)1 sequence of NYTMA (SEQ ID NO:11); a CDR2 sequence of VVSRGGGATDYADSVKG (SEQ ID NO:12); and a CDR3 sequence of GTDLSYYYSTKKWAY (SEQ ID NO:13); and b) evaluating the expression levels of the protein candidates; or c) evaluating the biophysical properties of the protein candidates; or d) evaluating the binding kinetics of the protein candidate; or e) any combination of steps b) to d) above,wherein the protein anchor binds to a specific coating thus capturing the protein candidates and facilitating the evaluation of expression levels, biophysical characteristics and binding kinetics; and wherein the specific coating is bovine serum albumin.