发明名称 |
Verbesserte Methoden um Säugetierzellen in vitro zu züchten |
摘要 |
The invention relates to a method for reduction of glucose consumption and/or lactate production during cultivation of animal cells, characterized in that cultivation is performed in the presence of a bi- or tricarbonic acid or a salt thereof at a concentration of about 1 to 50 mmol/l, whereby in the case where said di- or tricarbonic acid is citric acid or citrate this amount of said citric acid or citrate is not bound in chelate complex with iron or another transition metal ion. |
申请公布号 |
DE60301510(T2) |
申请公布日期 |
2006.06.14 |
申请号 |
DE2003601510T |
申请日期 |
2003.03.04 |
申请人 |
F. HOFFMANN-LA ROCHE AG, BASEL |
发明人 |
BEHRENDT, ULRICH;EBERHARDT, HORST;SZPERALSKI, BERTHOLD |
分类号 |
C12N5/06;C12N5/00 |
主分类号 |
C12N5/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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