MODIFIED POLYNUCLEOTIDES FOR THE PRODUCTION OF MEMBRANE PROTEINS

摘要

The invention relates to compositions and methods for the preparation, manufacture and therapeutic use of polynucleotides, primary transcripts and mmRNA molecules.

主权项

1. An isolated polynucleotide comprising;
(a) a first region of linked nucleosides, said first region encoding a polypeptide of interest, said polypeptide of interest selected from the group consisting of SEQ ID NOs 8144-16131; (b) a first flanking region located at the 5′ terminus of said first region comprising;
(i) a sequence of linked nucleosides selected from the group consisting of the native 5′ UTR of any of the nucleic acids that encode any of SEQ ID NOs 8144-16131, SEQ ID NOs: 1-4 and functional variants thereof; and(ii) at least one 5′ terminal cap; (c) a second flanking region located at the 3′ terminus of said first region comprising;
(i′) a sequence of linked nucleosides selected from the group consisting of the native 3′ UTR of any of the nucleic acids that encode any of SEQ ID NOs 8144-16131, SEQ ID NOs 5-21 and functional variants thereof; and(ii′) a 3′ tailing sequence of linked nucleosides.