发明名称 |
METHOD OF PREPARING LUNG ALVEOLAR EPITHELIAL TYPE II CELLS DERIVED FROM EMBRYONIC STEM CELLS |
摘要 |
A method of preparing a population of in vitro cultured cells of alveolar epithelial type II (ATII) cell lineage derived from at least one embryonic stem cell is disclosed which comprises (a) culturing said at least one embryonic stem cell in vitro in a medium comprising Matrigel(R), to produce differentiated cells without formation of an embryonic body, wherein at least some of the differentiated cells are of ATII cell phenotype; (b) identifying the differentiated cells of ATII cell phenotype by detecting expression of at least one biomarker of ATII cells; (c) isolating the differentiated cells having ATII cell phenotype; and (d) cloning the isolated cells to produce a population of cells having ATII cell phenotype. The resulting cells are preferably >99% pure ATII phenotype lineage and are potentially useful therapeutically for treating lung injury and disease.
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申请公布号 |
US2010021443(A1) |
申请公布日期 |
2010.01.28 |
申请号 |
US20080527969 |
申请日期 |
2008.02.21 |
申请人 |
BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM |
发明人 |
WETSEL RICK A.;WANG DACHUN |
分类号 |
A61K35/12;C12N5/071;C12Q1/02 |
主分类号 |
A61K35/12 |
代理机构 |
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