发明名称 |
Methods for differentially detecting a multimeric form from a monomeric form of a multimer-forming polypeptide through three-dimensional interactions |
摘要 |
The present invention relates to a method for differentially detecting a multimeric form from a monomeric form of a multimer-forming polypeptide in a biosample, which comprises the steps of: (a) preparing a carrier-capturing antibody conjugate by binding a capturing antibody to the surface of a solid phase carrier in a three dimensional manner, wherein the capturing antibody is capable of recognizing an epitope on the multimer-forming polypeptide; (b) preparing a detection antibody, wherein an epitope recognized by the detection antibody is present at a position in the multimer-forming polypeptide to cause a steric hindrance by the capturing antibody bound to its epitope to prevent the binding of the detection antibody to the multimer-forming polypeptide; (c) contacting simultaneously the carrier-capturing antibody conjugate and the detection antibody to the biosample; and (d) detecting the formation of a carrier-capturing antibody-multimeric form-detection antibody complex.
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申请公布号 |
US2010021943(A1) |
申请公布日期 |
2010.01.28 |
申请号 |
US20070297212 |
申请日期 |
2007.04.20 |
申请人 |
PEOPLEBIO, INC. |
发明人 |
AN SEONG SOO ALEXANDER;LIM KUN TAEK;OH HYUN JUNG |
分类号 |
G01N33/53;G01N33/543;G01N33/545;G01N33/553;G01N33/566 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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