<i>Thermus scotoductus </i>nucleic acid polymerases

摘要

The invention provides nucleic acids and polypeptides for a nucleic acid polymerase from a thermophilic organism, Thermus scotoductus. The invention also provides methods for using these nucleic acids and polypeptides.

主权项

1. An isolated derivative nucleic acid polymerase comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO:14, wherein said amino acid sequence comprises an Asp in place of Gly at a position corresponding to amino acid position 46 of SEQ ID NO:13, wherein said amino acid sequence further comprises an Arg at a position corresponding to amino acid position 250 of SEQ ID NO:13, and wherein said derivative nucleic acid polymerase has decreased 5′-3′ exonuclease activity compared to a wild type nucleic acid polymerase comprising SEQ ID NO:14.