Method for Identifying Drug Resistance Related Mutations

摘要

The present invention provides a method for detecting low frequency drug resistant mutations using ultra deep amplicon sequencing. Multiple selection criteria based on biological relevance were applied to filter out low frequency background noise and lead to reliable identification of drug resistance related mutations. A method to predict the occurrence of drug resistance during the treatment is also provided.

主权项

1. A method of identifying a drug resistance-related mutation, comprising comparing variant frequencies of a single nucleotide mutation in genomic DNA from a patient before drug treatment and at different time points during the drug treatment, wherein the single nucleotide mutation with increased variant frequency is the drug resistance-related mutation.