Composition for Enhancing Transgene Expression in Eukaryotic Cells and Method for Enhancing Production of a Target Protein Encoded by a Transgene

摘要

Production of DNA vectors with the inserted gene of a target protein and the production of recombinant protein in eukaryotic cell cultures is disclosed. A composition for the intensive production of target protein in eukaryotic cells comprises a DNA vector with the inserted gene of a target protein and an agonist of cell receptors belonging to the pattern recognition receptor (PRR) family selected from the following agonists: TLR2, or TLR4, or TLR5, or TLR7, or TLR8, or TLR9, or NOD1 receptor, or NOD2 receptor, used in an optimal ratio. TLR2 is a lipoteichoic acid. TLR2 is a lipopeptide. TLR4 can be either bacterial lipopolysaccharide or acidic peptidoglycan (APG) having a molecular weight of 1200-40000 kDa. TLR5 is flagellin TLR7 is either imiquimod or CL097, an imidazoquinoline derivative. TLR8 is either imiquimod or CL097, an imidazoquinoline derivative. TLR9 is either oligonucleotide CpG ODN 1826 or oligonucleotide CpG ODN 2006.

主权项

1. A composition for intensive production of target protein in eukaryotic cells, the composition comprising a DNA vector with an inserted gene of a target protein, and an agonist of cell receptors from a class of PRR—pattern recognition receptors, the antagonist being selected from the following agonists: TLR2 agonists, or TLR4 agonists, or TLR5 agonists, or TLR7 agonists, or TLR8 agonists, or TLR9 agonists, or NOD1 receptor agonists, or NOD2 receptor agonists, used in optimal ratios.