HEMOGLOBIN A1c MEASUREMENT METHOD AND MEASUREMENT KIT

摘要

This invention provides an amadoriase that acts on the β-chain of hemoglobin A1c (HbA1c) and generates hydrogen peroxide, a method for measurement of HbA1c using such amadoriase, and a reagent kit for measurement of HbA1c using such amadoriase. The method for measurement of HbA1c involves the use of an amadoriase that has specific activity of 0.1 U/mg or greater to αF6P and oxidizes the HbA1c β-chain amino terminus so as to generate hydrogen peroxide, and the reagent kit for measurement of HbA1c comprises such amadoriase. The method and the kit for measurement of HbA1c enable quantification of HbA1c to be performed rapidly, simply, and accurately.

主权项

1. A method for measurement of hemoglobin A1c in a sample comprising allowing an amadoriase capable of acting directly on hemoglobin A1c to act on a sample and measuring the resulting amount of hydrogen peroxide generated or oxygen consumed in the reaction.