| 摘要 |
The present invention discloses a method and a test element (110) for electrochemically detecting at least one analyte in a sample (126) of a body fluid, wherein the method comprises the following steps: a) providing at least one test element (110), wherein the test element (110) comprises at least one first electrode (112) contacting a test chemistry (122) and at least one second electrode (114), wherein the test chemistry (122) is or comprises a redox mediator in an oxidized form, wherein, in an absence of the sample (126), a surface (120) of the second electrode (114) adapted to contact the sample (126) of the body fluid consists of silver metal; b) contacting both the first electrode (112) and the second electrode (114) with the sample (126) of the body fluid comprising the chloride ions; c) applying a first voltage (Vi) between the first electrode (112) as a cathode and the second electrode (114) as an anode for a time and with a voltage sufficient for forming a layer of silver chloride at the surface (120) of the second electrode (114), wherein the oxidized form of the redox mediator is transformed into a reduced form; d) applying a second voltage (V2) between the first electrode (112) as the anode and the second electrode (114) as the cathode; and e) determining an electrical signal between the first electrode (112) and the second electrode (114), whereby the analyte in the sample (126) of the body fluid comprising the chloride ions is detected, wherein detecting the analyte comprises reoxidizing the redox mediator. Further, the present invention discloses a method for producing the test element (110), a method for generating a layer of silver chloride at a surface of an electrode of the test element (110), and a system for determining at least one property of the sample (126) of the body fluid, wherein the system comprises the test element (110). |
