Human cellular models with biosensors

摘要

This invention provides biosensors, cell models, and methods of their use for monitoring heme, oxygen or ATP. Biosensors can include targeting domains, sensing domains and reporting domains. Biosensors can be introduced into cells reprogrammed to represent experimental or pathologic cells of interest. Model cells expressing the biosensors can be contacted with putative bioactive agents to determine possible activities.

主权项

1. An oxygen sensor peptide construct comprising:
A binding domain which binds to heme; and, a fluorescent reporter domain, wherein the fluorescent reporter is adapted to fluoresce at wavelengths in the range from 500 nm to 1400 nm; wherein the fluorescent reporter domain is adapted to change fluorescent emissions characteristics in response to binding of the heme-binding domain to heme.