METHODS FOR PREDICTING OVERALL SURVIVAL OF CANCER PATIENTS BASED ON NUMBERS OF CIRCULATING TUMOR CELLS

摘要

Cytokeratin (CK)+/CD45−/DAPI+ cells in the blood of cancer patients have been considered by most as circulating tumor cells (CTCs). Different methods of isolating CTCs results in a wide range of numbers and subgroups of CTCs from same patient. As a result, the clinical significance of the number of CTCs becomes cloudy. Provided herein is methodology to categorize CTCs into morphologically distinct subpopulations, and to use one of the subpopulations in methods for predicting overall survival of a patient having cancer.

主权项

1. A method for predicting overall survival of a patient having cancer, comprising enumerating pathologically-defined circulating tumor cells (PDCTCs) in a blood sample from the patient, wherein when five or more PDCTCs are present in 7.5 ml of blood, the overall survival of the patient is predicted to be lower than a patient having cancer with four or less PDCTCs present in 7.5 ml of blood, and wherein the PDCTCs are cytokeratin (CK) 8, 18, 19+, CD45−, DAPI+ cells, possess a malignant nucleus, and display a filamentous CK pattern.