DETECTION OF NUCLEIC ACID MOLECULES USING NANOPORES AND COMPLEXING MOIETIES

摘要

The present disclosure provides methods and systems for assaying the presence of a target nucleic acid molecule in a sample having or suspected of having the target nucleic acid molecule. A method of assaying the presence of the target nucleic acid molecule comprises facilitating the flow of the sample through at least one nanopore in a membrane disposed adjacent or in proximity to an electrode that is adapted to detect a current or change thereof upon movement of a complex having the target nucleic acid molecule coupled to the complexing moiety through the at least one nanopore. Next, the current or change thereof is measured with the electrode. The complex in the sample is detected from the current or change thereof, thereby assaying the presence of the target nucleic acid molecule in the sample.