<p num="1"><br/>The present invention relates to a method for purifying bacterial cytolysins <br/>such as <br/>pneumococcal pneumolysin. A single chromatography step produces excellent <br/>purification of <br/>the cytolysin by binding soluble aggregated cytolysin to a hydrophobic <br/>interaction <br/>chromatography material in the presence of detergent and high salt.<br/>
