FACTOR VII POLYPEPTIDES THAT ARE MODIFIED AND USES THEREOF

摘要

Nucleic acid encoding modified factor VII polypeptides, vectors and cells containing the nucleic acid, uses of the nucleic acids, methods of making the encoded polypeptides, and methods of treatment are provided. The encoded modified FVII polypeptides include Factor VIIa and other forms of Factor VII. Among the encoded modified FVII polypeptides provided are those that have altered activities, typically altered procoagulant activity, including increased procoagulant activities.

主权项

1. A nucleic acid molecule encoding a modified factor VII (FVII) polypeptide that comprises amino acid replacements at positions corresponding to positions 286 and 298 in a FVII polypeptide having the sequence of amino acids set forth in SEQ ID NO:3, wherein:
the amino acid replacement at position 286 is Arg (R), and the amino acid replacement at position 298 is Gln (Q); the modified FVII polypeptide, when in an activated form, exhibits procoagulant activity; and the amino acid sequence of the modified FVII polypeptide has at least 90% sequence identity with a polypeptide having the sequence of amino acids set forth in any of SEQ ID NOS:1-3.