MICROORGANISM FOR METHIONINE PRODUCTION WITH IMPROVED METHIONINE SYNTHASE ACTIVITY AND METHIONINE EFFLUX

摘要

The present application is related to a recombinant microorganism optimised for the fermentative production of methionine and/or its derivatives, wherein in said recombinant microorganism, the cobalamin-dependent methionine synthase activity and the methionine efflux are enhanced. The application is also related to a method for optimizing the fermentative production of methionine and/or its derivatives comprising the steps of: c. culturing a recombinant microorganism wherein in said microorganism, the cobalamin-dependent methionine synthase activity and the methionine efflux are enhanced, in an appropriate culture medium comprising a fermentable source of carbon and a source of sulphur, and d. recovering methionine and/or its derivatives from the culture medium.

主权项

1. A recombinant microorganism optimised for the fermentative production of methionine and/or its derivatives, wherein in said recombinant microorganism, the expression of metH from E. coli, and optionally the expression of the genes fldA and fpr from E. coli or their homologous genes from C. glutamicum are enhanced and the genes ygaZH from E. coli, or the genes brnFE from C. glutamicum or their homologous genes are overexpressed.