System for imaging captured cells

摘要

A system for imaging captured cells comprising: an illumination module configured to illuminate a target object; a platform configured to position the target object in relation to the illumination module; a filter module configured to filter light transmitted to the target object and/or to filter light received from the target object, an optical sensor configured to receive light from the target object and to generate image data; and a focusing and optics module configured to manipulate light transmitted to the optical sensor. The system can further comprise one or more of: a control system configured to control at least one of the illumination module, the platform, the focusing and optics module, the filter module, and the optical sensor; a tag identifying system configured to identify and communicate tag information from system elements; a thermal control module configured to adjust temperature parameters of the system; and an image stabilization module.

主权项

1. A system for detecting biological sample features comprising:
a platform including a set of guides that receive a set of imaging substrates with a set of biological samples, wherein the set of guides is distributed about the platform in a first circular array; a set of filter modules superior to the platform, each of the set of filter modules including an excitation filter, an emission filter, and a dichroic mirror; a first light source, inferior to the platform, configured to transmit light toward a biological sample of the set of biological samples from a first direction; a second light source configured, in a first configuration, to transmit light through the excitation filter of a first of the set of filter modules, to be reflected from the dichroic mirror of the first of the set of filter modules, and to reach the biological sample from a second direction opposed to the first direction; a lens, coupled to a lens selector, that focuses light from the excitation filter onto a target object of the biological sample, and transmits light from the target object, through the dichroic mirror and the emission filter, toward an optical sensor; a platform control module configured to 1) rotate the platform about a first axis, associated with the first circular array and perpendicular to a broad surface of the platform and 2) translate the platform along a direction perpendicular to the first axis, into a first set of configurations that align each guide of the set of guides between the first light source and the lens; a filter stage, situated superior to the platform and wherein the set of filter modules is distributed about the filter stage in a second circular array, configured to rotate the set of filter modules about a second axis, parallel to the first axis, into a second set of configurations that aligns each of the set of filter modules to receive light from the second light source and to transmit light toward the optical sensor; and an optics manipulation module, including a translation stage coupled to an optical shaft concentrically aligned with the lens by way of the lens selector, configured to vertically translate the lens to facilitate focusing of the target object.