IMPROVED ACETYL-COA CARBOXYLASE VARIANTS

摘要

The disclosure relates to acetyl-CoA carboxylase (ACC) variants and host cells expressing them for the production of malonyl-CoA derived compounds including fatty acid derivatives. Further contemplated are methods of producing increased amounts of malonyl-CoA derived compounds and related cell cultures.

主权项

1. A variant biotin carboxyl carrier protein (BCCP) comprising at least one mutation in its amino acid sequence, wherein said variant BCCP comprises a polypeptide sequence selected from the group consisting of SEQ ID NOS: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88 and 90, and wherein expression of said variant BCCP confers to a recombinant cell an increased production of a malonyl-CoA-derived compound when compared to a corresponding wild type cell.