| 摘要 |
H:/sxd/Intrwovn/NRPortbl/DCC/SXD/l 1209626_ .docx-7/09/2016 The invention relates to a protein chip and kit for detecting abnormal des-carboxy-prothrombin (DCP) in serum and the preparing methods thereof, which belonging to the technology of protein detection. Said chip comprises at least one detection subarea on a matrix, wherein each said detection subarea is used to detect one serum sample and comprises a detection spot region and a control spot region, wherein the detection spot region comprises detection spots formed by DCP specific antibody solution and the control spot region comprises control spots formed by bovine serum albumin solution; the detection spots within the same detection spot region have the same volume of DCP specific antibody; the total volume of the DCP specific antibody solution is 3nl for each detection spot, which is formed by 6-10 sprays, with 300-500pl for each spraying and spotting. The protein chip and kit of the invention can accurately detect abnormal DCP, and have the advantages of high sensitivity, time saving, convenience, economy, etc. in clinical use. H:/xd/Interwoven/NRPortbl/DCC/SXD/l 1209639_I.docx-7/09/2016 0 * * 0 0 0 0* L10%BSA DCP Antibodies Figure 1 |
