| 摘要 |
A method of obtaining a stem cell is described, comprising obtaining a somatic cell, which cell contains a gene coding for a marker which is differentially expressed in (a) desired stem cells and (b) cells other than desired stem cells; obtaining an enucleated oocyte; transferring the nucleus of the somatic cell into the enucleated oocyte to form a transgenic oocyte; culturing the transgenic oocyte to produce a culture of cells derived from the transgenic oocyte and identifying a cell of the culture that expresses the marker, thus indicating that the nucleus of that cell has been reprogrammed. Also described are assays for factors that can reprogram nuclei and stem cells obtained directly or indirectly using the nuclear reprogramming methods described. |
