| 摘要 |
Methods and compositions are provided for producing at least one donor cell that has a genome identical to that of a host. In the subject methods, an embryo is first produced by combining a nucleus obtained from a cell of said host with a recipient cell, e.g., an enucleated oocyte. The resultant embryo is then matured under conditions sufficient to produce a non-sentient organism that includes the donor cell. Finally, the donor cell is harvested from the non-sentient organism for subsequent use. Also provided are kits for use in practicing the subject invention. The subject methods find use in a variety of different applications, including tissue and organ transplant applications.
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