RECOMBINANT ADENO-ASSOCIATED VIRUS COMPRISING ANTISENSE CDNAS OF VEGF-A, VEGF-B AND VEGF-C AND GENE THERAPEUTIC AGENT SPECIFIC TO LARGE INTESTINE CANCER, BLADDER CANCER AND/OR LUNG CANCER COMPRISING THE SAME

摘要

A recombinant adeno-associated virus comprising antisense cDNAs of VEGF-A(vascular endothelial growth factor-A), VEGF-B and VEGF-C and a gene therapeutic agent specific to large intestine cancer, bladder cancer and/or lung cancer comprising the same virus are provided to reduce proliferation of tumor cells by inhibiting expression of VEGF associated with angiogenesis which is necessary for proliferation and transition of tumor cells. The recombinant adeno-associated virus(rAAV) vector comprises an antisense cDNA of VEGF-A of SEQ ID NO:1, an antisense cDNA of VEGF-B of SEQ ID NO:4 and an antisense cDNA of VEGF-C of SEQ ID NO:7, wherein the rAAV vector is prepared by (a) transfecting an animal cell line with pAAV vector containing the antisense cDNA of VEGF-A of SEQ ID NO:1, the antisense cDNA of VEGF-B of SEQ ID NO:4 and the antisense cDNA of VEGF-C of SEQ ID NO:7, an AAV rep-cap plasmid DNA and an adenovirus helper plasmid, (b) culturing the transfected animal cell line, and (c) destroying the cultured animal cell line, and isolating and purifying the rAAV particle. The gene therapeutic agent specific to large intestine cancer, bladder cancer and/or lung cancer comprises the recombinant adeno-associated virus(rAAV) vector.